Wheat variety W050216N2

ABSTRACT

A wheat variety designated W050216N2, the plants and seeds of wheat variety W050216N2, methods for producing a wheat plant produced by crossing the variety W050216N2 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W050216N2 with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety W050216N2. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety W050216N2 and to wheat varieties or breeding lines produced by those methods.

FIELD OF INVENTION

This invention is in the field of wheat (Triticum aestivum L.) breeding,specifically relating to a wheat variety designated W050216N2.

BACKGROUND OF INVENTION

There are numerous steps involving significant technical humanintervention in the development of any novel, desirable plant germplasm.Plant breeding begins with the analysis and definition of problems andweaknesses of the current germplasm, the establishment of program goals,and the definition of specific breeding objectives. The next step isselection of germplasm that possess the traits to meet the programgoals. The goal is to combine in a single variety an improvedcombination of desirable traits from the parental germplasm. Thesetraits may include, but are not limited to higher seed yield, resistanceto diseases and/or insects, tolerance to drought and/or heat, alteredmilling properties, abiotic stress tolerance, improvements incompositional traits, and better agronomic characteristics.

Processes which lead to the final step of marketing and distribution cantake from approximately six to twelve years of significant technicalhuman intervention starting from the time the first cross is made.Therefore, development of new varieties is a time-consuming process thatrequires precise forward planning, efficient use of resources, and aminimum in changes of direction. Stable, high yielding wheat varietiesthat are agronomically sound and which maximize the amount of grainproduced on the land used are selected and developed to provide superiorwheat plant varieties. Significant human intervention is required.

Wheat is grown worldwide and is the most widely adapted cereal. Thereare five main wheat market classes. They include the four common wheat(Triticum aestivum L.) classes: hard red winter, hard red spring, softred winter, and white. The fifth class is durum (Triticum turgidum L.).Common wheats are used in a variety of food products such as bread,cookies, cakes, crackers, and noodles. In general the hard wheat classesare milled into flour used for breads and the soft wheat classes aremilled into flour used for pastries and crackers. Wheat starch is alsoused in the paper industries, as laundry starches, and in otherproducts.

SUMMARY OF THE INVENTION

In certain embodiments, a plant, plant part, seed, or plant cell ofwheat variety W050216N2 is provided, representative seed of varietyW050216N2 having been deposited with the ATCC.

In certain embodiments, wheat seed is provided from the cross of theplant or plant part of wheat variety W050216N2 with a different wheatplant or plant part. Plants and plant parts grown from the seed of thecross are also provided. Methods for producing different wheat plantsare provided in which plant breeding techniques are applied to the wheatplant or plant part grown from the seed of the cross.

In certain embodiments, methods for producing progeny seed and theprogeny seed so made are provided in which a wheat plant produced bygrowing a seed of the cross of wheat variety W050216N2 with a differentwheat plant or plant part is then crossed to a plant of wheat varietyW050216N2. Methods and backcrossed seed are also provided in which theprogeny seed is grown and crossed to a plant wheat variety W050216N2 toproduce backcrossed seed.

In certain embodiments, methods for producing double haploid wheatplants are provided in which a wheat plant produced by growing a seed ofthe cross of wheat variety W050216N2 with a different wheat plant orplant part is then crossed with another plant to form haploid cells. Thechromosomes of the haploid cells are doubled to form double haploidcells which are grown into a double haploid wheat plant or plant part.

In certain embodiments, methods for cleaning, conditioning, or applyinga seed treatment to the seed of wheat variety W050216N2 are provided.

In certain embodiments, methods of milling the seed of wheat varietyW050216N2 and the flour produced from such milling is provided. Theflour may include a cell of wheat variety W050216N2.

In certain embodiments, a tissue culture of cells is provided which areproduced from the plant, plant part, seed or cell of wheat varietyW050216N2. Plants and plant parts regenerated from the tissue cultureare also provided.

In certain embodiments, wheat plants are provided which plants include atransgene and which were produced by transforming the plant, plant part,seed or cell of wheat variety W050216N2.

In certain embodiments, a plant, plant part, seed, or plant cell ofwheat variety W050216N2 further comprising a locus conversion isprovided. The plant, plant part, seed, or plant cell may have other thanthe locus conversion essentially all of the morphological andphysiological characteristics of wheat variety W050216N2. The locusconversion may confer a trait selected from male sterility, abioticstress tolerance, altered phosphorus, altered antioxidants, alteredfatty acids, altered essential amino acids, altered carbohydrates,herbicide resistance, insect resistance, disease resistance or acombination thereof.

In certain embodiments, methods for producing a wheat plant are providedin which plant breeding techniques are applied to a wheat plant grownfrom seed of wheat variety W050216N2 comprising a locus conversion, orto a plant grown from seed of a cross of such a wheat plant to adifferent wheat plant.

DETAILED DESCRIPTION

The present invention relates to a new and distinctive wheat (Triticumaestivum L.), variety designated W050216N2, which has been the result ofyears of careful breeding and selection in a comprehensive wheatbreeding program.

The modified pedigree selection method of breeding was used to derivethis line from elite germplasm. The first cross was made in 2004 andbreeding and selection continued until 2013. W050216N2 represents asignificant advancement in elite germplasm adapted to the United States.

Field crops are bred through techniques that take advantage of theplant's method of pollination, such as self-pollination, sib-pollinationor cross-pollination. As used herein, the term cross-pollinationincludes pollination with pollen from a flower on a different plant froma different family or line and does not include self-pollination orsib-pollination. Wheat plants (Triticum aestivum L.), are recognized tobe naturally self-pollinated plants which, while capable of undergoingcross-pollination, rarely do so in nature. Thus intervention for controlof pollination is needed for the establishment of superior varieties.

Provided are methods of producing progeny with a new combination ofgenetic traits by cross pollinating one wheat plant with another byemasculating flowers of a designated female plant and pollinating thefemale parent with pollen from the designated male parent. Suitablemethods of cross-pollination of wheat plants are described, for example,in U.S. Pat. No. 8,809,654, which is herein incorporated by reference,but other methods can be used, or modified, as is known to those skilledin the art.

A cross between two different homozygous lines produces a uniformpopulation of hybrid plants that may be heterozygous for many gene loci.A cross of two heterozygous plants each that differ at a number of geneloci will produce a population of plants that differ genetically andwill not be uniform. Regardless of parentage, plants that have beenself-pollinated and selected for type for many generations becomehomozygous at almost all gene loci and produce a uniform population oftrue breeding progeny. The term “homozygous plant” is hereby defined asa plant with homozygous genes at 95% or more of its loci.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of variety used commercially (e.g., F1 hybrid variety, purelinevariety, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection can be based on meanvalues obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.For example, pedigree breeding, backcross breeding, single seed descent,and bulk breeding, which are each described in U.S. Pat. No. 8,809,654(incorporated herein by reference), can be used. Each wheat breedingprogram may include a periodic, objective evaluation of the efficiencyof the breeding procedure. Evaluation criteria vary depending on thegoal and objectives, but may include gain from selection per year basedon comparisons to an appropriate standard, overall value of the advancedbreeding lines, and number of successful varieties produced per unit ofinput (e.g., per year, per dollar expended, etc.).

Various recurrent selection techniques can be used to improvequantitatively inherited traits controlled by numerous genes. The use ofrecurrent selection in self-pollinating crops depends on the ease ofpollination and the number of hybrid offspring from each successfulcross. Recurrent selection can be used to improve populations of eitherself- or cross-pollinated crops. A genetically variable population ofheterozygous individuals is either identified or created byintercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued. Plantsfrom the populations can be selected and selfed to create new varieties.

Wheat variety W050216N2 can be used as the female or the male parent inbiparental crosses in order to develop new and valuable wheat varieties.Wheat normally self-pollinates in nature. Wheat cross-pollination can beachieved by emasculating a designated female plant and pollinating thefemale plant with pollen from the designated male parent.

In order to cross pollinate one wheat plant with another to produceprogeny with a new combination of genetic traits, a method ofcross-pollination is employed. Cross-pollination is known to thoseskilled in the art. Wheat cross-pollination is achieved by emasculatingflowers of a designated female plant and pollinating the female parentwith pollen from the designated male parent. The following method wasemployed to cross-pollinate the wheat plants, but other methods can beused, or modified, as is known to those skilled in the art.

The designated female wheat plant is emasculated before its anthers shedpollen to avoid self-pollination. Emasculation is done by selecting animmature spike on the designated female parent plant that has notstarted to bloom and shed any viable pollen. Each spike consists of aseries of spikelets composed of florets which each contain one ovarywith a feathery stigma and three anthers. Typically all but the twoprimary florets are removed from each spikelet by using tweezers. Theglumes of each remaining floret can be trimmed back about 50% usingscissors to expose the immature anthers. The tweezers are used to spreadthe glumes slightly open while at the same time surrounding the anthers.The anthers can then be removed by gently grabbing and pulling them outof the flower with the tweezers in an upward motion. With skill, allthree anthers can be removed at once, but this must be confirmedvisually before moving to the next flower. Repeated attempts to removeany remaining anthers increases the risk of damage to the stigma andovary and greatly reduce the frequency of cross-pollination. After allthe florets are emasculated on a spike, it is covered with a cellophanebag to prevent pollination with stray pollen from surrounding plants.One to three days after the female spike is emasculated a mature spikethat is shedding pollen is selected from the designated male plant forcross-pollination using the approach method. The stem of the male spikeis cut off at least one foot below the spike and typically the glumes ofall the spikelets are trimmed back with scissors to encourage antherextrusion during pollination. The stem of the male spike is placed in atest tube full of water, which is attached to a stick implanted besidethe emasculated female spike. The male spike is placed above theemasculated female spike(s) in the same cellophane bag and it ispermitted to shed pollen naturally over the next several days. Bywaiting a few days after emasculation, one can ensure that no anthers orviable pollen has remained in the female spike and the stigmas becomemore receptive to cross-pollination.

Emasculated female spikes that are effectively cross-pollinated by thedesignated male parent will typically set 10-30 seeds per spike.Depending on the breeding objectives, one to five spikes are typicallycross-pollinated for each cross. Spikes from the cross are handharvested and the F1 seed from the spikes are advanced to the F1generation. The F1 plants can be used for used for subsequentcross-pollination or they can be advanced to the F2 generation forselection and further advancement. For the F2 grow out, 2500 to 3500seeds are typically planted.

Plant breeding methods may include use of molecular markers, includingtechniques such as Starch Gel Electrophoresis, Isozyme Electrophoresis,Restriction Fragment Length Polymorphisms (RFLPs), Randomly AmplifiedPolymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction(AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence CharacterizedAmplified Regions (SCARs), Amplified Fragment Length Polymorphisms(AFLPs), Simple Sequence Repeats (SSRs), Single Nucleotide Polymorphisms(SNPs) and Quantitative Trait Loci (QTL) mapping.

Molecular markers can also be used during the breeding process for theselection of qualitative traits. For example, markers closely linked toalleles or markers containing sequences within the actual alleles ofinterest can be used to select plants that contain the alleles ofinterest during a backcrossing breeding program. The markers can also beused to select for the genome of the recurrent parent and against themarkers of the donor parent. Using this procedure can minimize theamount of genome from the donor parent that remains in the selectedplants. It can also be used to reduce the number of crosses back to therecurrent parent needed in a backcrossing program. The use of molecularmarkers in the selection process is often called Genetic Marker EnhancedSelection.

The production of double haploids can also be used for the developmentof homozygous lines in the breeding program and in the production of,for example, hybrid wheat. In an embodiment, the variety is used in theproduction of hybrid wheat. Double haploids are produced by the doublingof a set of chromosomes (1N) from a heterozygous plant to produce acompletely homozygous individual. This can be advantageous because theprocess omits the generations of selfing needed to obtain a homozygousplant from a heterozygous source. Hybrid wheat can be produced, forexample, with the help of cytoplasmic male sterility, nuclear geneticmale sterility, chemicals or a combination thereof.

A breeder uses various methods to help determine which plants should beselected from the segregating populations and ultimately which lineswill be used for commercialization. In addition to the knowledge of thegermplasm and other skills the breeder uses, a part of the selectionprocess is dependent on experimental design coupled with the use ofstatistical analysis. Experimental design and statistical analysis canbe used to help determine which plants, which family of plants, andfinally which lines are significantly better or different for one ormore traits of interest. Experimental design methods can be used tocontrol error so that differences between two lines can be moreaccurately determined. Statistical analysis includes the calculation ofmean values, determination of the statistical significance of thesources of variation, and the calculation of the appropriate variancecomponents. Five and one percent significance levels are customarilyused to determine whether a difference that occurs for a given trait isreal or due to the environment or experimental error.

Plant breeding is the genetic manipulation of plants. The goal of wheatbreeding is to develop new, unique and superior wheat varieties. Inpractical application of a wheat breeding program, the breeder initiallyselects and crosses two or more parental lines, followed by repeatedselfing and selection, producing many new genetic combinations. Thebreeder can theoretically generate billions of different geneticcombinations via crossing, selfing and mutations. Each year, the plantbreeder selects the germplasm to advance to the next generation. Thisgermplasm is grown under unique and different geographical, climatic andsoil conditions and further selections are then made during and at theend of the growing season.

Plants are tested to detect major faults and establish the level ofsuperiority or improvement over current varieties. Research anddevelopment, breeding and testing processes, which lead to the finalstep of marketing and distribution, can take from six to twelve yearsfrom the time the first cross is made. Therefore, development of newvarieties is a time-consuming process that requires precise forwardplanning, efficient use of resources, and a minimum of changes indirection.

According to the invention, there is provided a novel wheat variety,designated W050216N2 and processes for making W050216N2. This inventionrelates to seed of wheat variety W050216N2, to the plants of wheatvariety W050216N2, to plant parts of wheat variety W050216N2, and toprocesses for making a wheat plant that comprise crossing wheat varietyW050216N2 with another wheat plant. This invention also relates toprocesses for making a wheat plant containing in its genetic materialone or more traits introgressed into W050216N2 through backcrossconversion and/or transformation, and to the wheat seed, plant and plantparts produced thereby. This invention also relates to the creation ofvariants by mutagenesis or transformation of wheat W050216N2. Thisinvention further relates to a hybrid wheat seed, plant or plant partproduced by crossing the variety W050216N2 or a locus conversion ofW050216N2 with another wheat variety.

Wheat varieties that are highly homogeneous, homozygous and reproducibleare useful as commercial varieties. There are many analytical methodsavailable to determine the homozygotic stability, phenotypic stability,and identity of these varieties.

In some embodiments, data can be collected from the observation ofphenotypic traits over the life of the wheat plants in fieldexperiments. Phenotypic characteristics observed can include traits suchas seed yield, head configuration, glume configuration, seedconfiguration, lodging resistance, disease resistance, maturity. Thegenotype of a plant can also be examined. There are manylaboratory-based techniques available for the analysis, comparison andcharacterization of plant genotype including, without limitation, GelElectrophoresis, Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats(SSRs) which are also referred to as Microsatellites, and SingleNucleotide Polymorphisms (SNPs). Gel electrophoresis is particularlyuseful in wheat. Wheat variety identification can occur, for example,through electrophoresis of gliadin, glutenin, albumin and globulin, andtotal protein extracts.

Wheat variety W050216N2 has shown uniformity and stability for alltraits, as described in the following variety description information.It has been self-pollinated a sufficient number of generations, withcareful attention to uniformity of plant type to ensure homozygosity andphenotypic stability. The line has been increased with continuedobservation for uniformity. No variant traits have been observed or areexpected in W050216N2, as described in Table 1B.

Wheat variety W050216N2 is a common, soft red winter wheat. VarietyW050216N2 demonstrates excellent yield potential with very good testweight, very good resistance to Fusarium head blight (scab) andexcellent leaf rust and stripe rust resistance. Variety W050216N2 hasmedium maturity relative to other varieties in the primary region ofadaptation. It has shown adaptation to the northern soft wheat regionsbased on tests conducted in Illinois, Indiana, Michigan, Missouri, Ohio,and Ontario, Canada.

Wheat variety W050216N2 was developed by from a cross between threehomozygous lines 25R62, 25R63, and W990479G1. Wheat variety W050216N2,being substantially homozygous, can be reproduced by planting seeds ofthe line, growing the resulting wheat plants under self-pollinating orsib-pollinating conditions, and harvesting the resulting seed, usingtechniques familiar to the agricultural arts.

Wheat variety W050216N2 is a soft red winter wheat (Triticum aestivumL.) derived from a three parent cross made in 2004 as follows:

-   -   25R62/25R63//W99047901

The single cross 25R62/25R63 was made during the 2004 spring greenhousecycle. The final cross was made in the 2004 fall greenhouse cycle. Thisand the subsequent breeding history of W050216N2 are described below.

Year Generation 2004 Final cross Final cross made in greenhouse 2005 F1F1 transplanted into field nursery 2006 F2 F2 bulk populations grown attwo locations 2007 F3 F3 headrows from F2 plant selections grown at twolocations 2008 F4 F4 bulk from selected F3 headrows grown at twolocations 2009 F5 F5 bulk from selected F4 bulk grown at two locations2010 F6 F6 headrows from F5 plant selections grown at two locations 2011F7 F7 preliminary yield testing 2012 F8 F8 advanced yield testing andheadrow purification/increase 2013 F9 F9 elite yield testing and headrowpurification/increase

During the process of development, the plant populations as well asindividual plants are evaluated for general health, agronomics, andstability at many stages. These evaluations typically include, but arenot limited to, one or more of the following characteristics: plantarchitecture traits such as seedling coleoptile length, coleoptile color(presence of anthocyanin), juvenile plant growth habit, tillering, plantheight, straw strength or lodging, flag leaf carriage at boot stage,leaf width and length, glaucosity of stems, leaves and spikes,pubescence of leaves and spikes, spike shape, spike density, spikeawnedness, and plant color through-out stages of growth; plant growthcharacteristics, such as vernalization requirement, date for first stemjoint emergence, heading date, flowering date, physiological maturitydate and harvest maturity; tolerance to weather conditions, such as coldtolerance, resistance to heaving, tolerance to wet soils and standingwater, drought and heat tolerance; and grain characteristics, such asgrain yield, test weight, 1000 kernel weight, grain moisture, graincolor, grain shape, grain protein, flour milling yield and bakingcharacteristics.

During its development, wheat variety W050216N2 was assayed and/orplanted in field trials and evaluated for a variety of traits and/orcharacteristics as compared to check varieties. The property(s) ofappropriate check varieties include but are not limited to varietieswith a similar relative maturity, varieties known to be susceptible toone or more particular diseases, insect, pathogen, field condition,weather condition, soil type or condition, and/or crop managementpractice, varieties known to be tolerant or resistant to one or moreparticular diseases, insect, pathogen, field condition, weathercondition, soil type or condition, and/or crop management practice,varieties comprising one or more particular marker locus, and/orvarieties derived from another appropriate variety or having aparticular pedigree. Appropriate choice of check varieties forcomparison assures an appropriate baseline and valid qualitative orquantitative assessment of any test varieties.

Throughout the course of the development of W050216N2, the plants can betested for various traits including, but not limited to grain yield,test weight, heading date, harvest maturity, plant height, strawstrength, pre-harvest sprout tolerance, resistance levels to leaf rust,stripe rust, tan spot, Septoria tritici blotch, Stagnospora nodorumblotch, powdery mildew, Fusarium (scab), wheat yellow mosaic virus andsoilborne mosaic virus, and grain characteristics such as flour yield,flour protein, and baking characteristics.

The cultivar W050216N2 was bred and selected using a modified pedigreeselection method for any and all of the following characteristics in thefield environment: disease resistance, plant type, plant height, headtype, straw strength, maturity, grain yield, test weight, and millingand baking characteristics. W050216N2 has been shown to be uniform andstable since the 7^(th) generation, or for the last 3 generations.W050216N2 has shown no variants other than what would normally beexpected due to environment.

Wheat variety W050216N2, being substantially homozygous, can bereproduced by planting seeds of the line, growing the resulting wheatplants under self-pollinating or sib-pollinating conditions, andharvesting the resulting seed, using techniques familiar to theagricultural arts.

When referring to area of adaptability, such term is used to describethe location with the environmental conditions that would be well suitedfor this wheat variety. Area of adaptability is based on a number offactors, for example: days to heading, winter hardiness, insectresistance, disease resistance, and drought resistance. Area ofadaptability does not indicate that the wheat variety will grow in everylocation within the area of adaptability or that it will not growoutside the area.

Northern area=States of DE, IL, IN, MI, MO, NJ, NY, OH, PA, WI andOntario, Canada

Mid-south=States of AR, KY, MO Bootheel and TN

Southeast=States of NC, SC, and VA

Deep South=States of AL, GA, LA, and MS

Table 1A lists common traits and a description of how the trait isscored.

TABLE 1A TRAIT DESCRIPTION & HOW SCORED HD DAT Heading Date in days pastJan. 1st); plot dated on the day when approximately 50% of the heads are50% out of the boot HGTIN Height (inches or centimeters); scored with ameasuring HGTCM stick after all genotypes fully extended; wheat gatheredaround stick and average distance to the top of the heads is noted; 2-3samplings per plot LF BLT Leaf Blight Complex; score based on amount ofinfection on flag and flag −1 leaves; typical scale: % of uninfectedleaf surface area flag flag −1 9 - 100% 100% 8 - 100% 75% 7 - 100% 50%6- >90% <50% 5 - 75-90% <25% 4 - 50-74% — 3 - 23-49% — 2 - 10-24% — 1 -0-9% — LF RST Leaf Rust; score based on amount of infection evident onflag leaves; typical scale: 9 - clean 8 - trace amounts 7 - <5% flagleaf area infected 6 - 6-10% flag leaf area infected 5 - 11-20% flagleaf area infected 4 - 21-30% flag leaf area infected 3 - 31-40% flagleaf area infected 2 - 41-50% flag leaf area infected 1 - over 50% flagleaf area infected MAT Maturity; used on larger, earlier generationtests in the place of heading date; scale based on maturity of knownchecks and will vary from year to year based on when the note is taken;typical scale: 9 - very late, boot not swelling when note is taken 8 -still in boot when note is taken 7 - splitting boot, will head two daysafter note is taken 6 - will head day after the note is taken 5 - headedon the day note is taken 4 - headed day before note taken 3 - headed twodays before note taken 2 - fully extended, some flowering visible 1 -extended and flowering Maturity may also be scored at physiologicalmaturity; typical scaler: 9- ready to be harvested 7- caryopse hard todivide 5- head yellowing an day note is taken 3- grain still at doughstage 1- head completely green PM Powdery Mildew; score based onseverity of infection and progression of the disease up the plant; scalebased on reaction of known checks with attention given to race changes;typical scale: 9 - clean 8 - trace amount low on plants 7 - slightinfection mostly low on plants 6 - moderate infection low on plants;trace amounts on flag −1 leaves 5 - moderate infection low on plants,moderate amounts on flag −1 leaves 4 - moderate infection through canopywith trace amounts evident on flag leaves 3 - severe infection throughcanopy with up to 25% infection on flag leaves 2 - severe infectionthrough canopy with up to 50% infection on flag leaves 1 - severeinfection; greater than 50% infection on flag leaves SB MV Soil BorneMosaic Virus; score based on amount of mottling, chlorosis, and/orstunting; scale based on reaction of known checks; typical scale 1 -severe stunting to the point of rosettes 2 - severe stunting 3 - verychlorotic with moderate stunting 4 - very chlorotic with mild stunting5 - moderate mottling with no stunting 6 - mottling evident 7 - mottlingbarely visible 8 - green, very little mottling 9 - green, no mottlingvisible SHTSC Shattering score. Scores are based on the amount of grainthat is visible in the spike just before harvest. 9 - grain no visiblein the spike, Glumes closed. 8 - Glumes slightly opened in <10% of thegrains. 7 - Glumes slightly opened in >10% of the grains. 6 - Glumesmoderately opened in <20% of the grains. 5 - Glumes moderately openedin >20% of the grains. 4 - Glumes completely opened in <30% of thegrains. 3 - Glumes completely opened in >30% of the grains. 2 - 20%-50%of the grain on the soil 1 - >50% of the grain on the soil. SS MVSpindle Streak Mosaic Virus; score based on amount of mottling andchlorosis; scale based on reaction of known checks; scale similar to SSMV with less emphasis on stunting ST EDG Straw Lodging; score based onamount of lodging; typical scale: 9 - still upright 8 - only slightleaning 7 - some leaning, no lodging 6 - moderate leaning, littlelodging 5 - up to 10% lodged 4 - 11-25% lodged 3 - 26-50% lodged 2 -51-75% lodged 1 - greater than 75% lodged STPRST Stripe rust. Striperust is an important disease that occurs most often in Europe. Theinfection may only affect the flag leaf, or it may attack the entireplant including the head. Two scales based on level of infectionincluded below: Score based on the amount of infection of the wholeplant! 9 - clean 8 - traces 7 - <5% plant infected 6 - 10% plantinfected 5 - 20% plant infected 4 - 40% plant infected 3 - 60% plantinfected 2 - 60% plant infected head rusted 1 - Plant not able toproduce kernel Score based on the amount and type of infection evidenton flag leaves: 9 - clean 8 - trace amounts (Chlorotic-necroticfreckles) 7 - <5% flag leaf area infected 6 - 6-10% flag leaf areainfected (chlorotic-necrotic stripes). 5 - 11-20% flag leaf areainfected (chlorotic-necrotic stripes). 4 - 21-30% flag leaf areainfected (chlorotic-necrotic stripes). 3 - 31-40% flag leaf areainfected (chlorotic-necrotic stripes). 2 - 41-50% flag leaf areainfected (some chlorosis). 1 - over 50% leaf area infected (nochlorosis). UNI Uniformity; used to determine how pure a line isgenerally at the F7 (pre-advanced) generation; typical scale: 9 - veryuniform in all aspects 8 - good uniformity 7 - fairly uniform, but someoff-types 6 - several off-types, but can be cleaned up with normalpurification procedures 5 - several off-types, will be a challenge toclean up with normal purification procedures 4 - considerable number ofoff-types; will need to be reselected to proceed as a pureline 3 - asmany as 25% off types; will need to be reselected 2 - as many as 50% offtypes; will need to be reselected 1 - more than 50% off types; what youhave here is a problem WNTHRD Winter Hardiness; score based on amount ofbrownback and kill; best scored at time of early spring regrowth;typical scale: 9 - very green, no brown-back 8 - green, slightbrown-back 7 - moderate brown-back 6 - hard brown-back, no kill 5 - hardbrown-back with less than 10% kill 4 - 11-25% kill 3 - 26-50% kill 2 -51-75% kill 1 - greater than 75% kill SC AB fusarium head scab; scorebased on visual evaluation of the percentage of scab infected heads on awhole plot basis with consideration given to both total heads affectedand severity of infection; typical scale: 9 - no scab infection 8 -trace amount (1-2%) with infections limited to individual spikelets 7 -up to 5% infection with most infection limited to less than 50% of thespike 6 - 5-15% of heads infected 5 - 15-30% of heads infected 4 -30-50% of heads infected 3 - 50-75% of heads infected 2 - 75-90% ofheads infected 1 - >90% of heads infected most genotypes scoring 5 orbelow would typically have the majority of the spike infected

TABLE 1B VARIETY DESCRIPTION INFORMATION W050216N2 1. KIND: 1 (1 =Common, 2 = Durum, 3 = Club, 4 = Other) 2. VERNALIZATION: 2 (1 = Spring,2 = Winter, 3 = Other) 3. COLEOPTILE ANTHOCYANIN: 1 (1 = Absent, 2 =Present) 4. JUVENILE PLANT GROWTH: 2 (1 = Prostrate, 2 = Semi-erect, 3 =Erect) 5. PLANT COLOR (boot stage): 2 (1 = Yellow-Green, 2 = Green, 3 =Blue-Green) 6. FLAG LEAF (boot stage): 2 (1 = Erect, 2 = Recurved) FLAGLEAF (boot stage): 2 (1 = Not Twisted, 2 = Twisted) FLAG LEAF (bootstage): 2 (1 = Wax Absent, 2 = Wax Present) 7. EAR EMERGENCE: 126 =Number of Days after Jan. 1 and Same day as 25R47 8. ANTHER COLOR: 1 (1= Yellow, 2 = Purple) 9. PLANT HEIGHT (from soil to top of head,excluding awns): 94 cm (Average) 8 cm Taller Than 25R47 10. STEM: A.ANTHOCYANIN: 1 (1 = Absent, 2 = Present) B. WAXY BLOOM: 2 (1 = Absent, 2= Present) C. HAIRINESS (last internode of rachis): 2 (1 = Absent, 2 =Present) D. INTERNODE: 1 (1 = Hollow, 2 = Semi-solid, 3 = Solid) E.PEDUNCLE: 3 (1 = Erect, 2 = Recurved, 3 = Semi-erect) F. AURICLEAnthocyanin: 2 (1 = Absent, 2 = Present) Hair: 2 (1 = Absent, 2 =Present) 11. HEAD (at maturity) A. DENSITY: 1 (1 = Lax, 2 = Middense, 3= Dense) B. SHAPE: 2 (1 = Tapering, 2 = Strap, 3 = Clavate, 4 = Other)C. CURVATURE: 3 (1 = Erect, 2 = Inclined, 3 = Recurved) D. AWNEDNESS: 4(1 = Awnless, 2 = Apically Awnletted, 3 = Awnletted 4 = Awned) 12.GLUMES (at Maturity): A. COLOR: 1 (1 = White, 2 = Tan, 3 = Other) B.SHOULDER: 2 (1 = Wanting, 2 = Oblique, 3 = Rounded, 4 = Square, 5 =Elevated, 6 = Apiculate) C. SHOULDER WIDTH: 2 (1 = Narrow, 2 = Medium, 3= Wide) D. BEAK: 3(1 = Obtuse, 2 = Acute, 3 = Acuminate) E. BEAK WIDTH:2 (1 = Narrow, 2 = Medium, 3 = Wide) F. GLUME LENGTH: 2 (1 = Short (ca.7 mm), 2 = Medium (ca. 8 mm), 3 = Long (ca.9 mm)) G. GLUME WIDTH: 2 (1 =Narrow (ca. 3 mm), 2 = Medium (ca. 3.5 mm), 3 = Wide (ca. 4 mm) H.PUBESCENCE: 1 (1 = Not Present 2 = Present) 13. SEED: A. SHAPE: 1 (1 =Ovate, 2 = Oval, 3 = Elliptical) B. CHEEK: 1 (1 = Rounded, 2 = Angular)C. BRUSH : 1 (1 = Short, 2 = Medium, 3 = Long) BRUSH: 1 (1 = NotCollared, 2 = Collared) D. CREASE: 2 (1 = Width 60% or less of Kernel, 2= Width 80% or less of Kernel, 3 = Width Nearly as Wide as Kernel)CREASE: 2 (1 = Depth 20% or less of Kernel, 2 = Depth 35%, or less ofKernel, 3 = Depth 50% or less of Kernel) E. COLOR: 3 (1 = White, 2 =Amber, 3 = Red, 4 = Other) F. TEXTURE: 2 (1 = Hard, 2 = Soft, 3 = Other)G. PHENOL REACTION: 3 (1 = Ivory, 2 = Fawn, 3 = Light Brown, 4 = DarkBrown 5 = Black) H. SEED WEIGHT: 37 g/1000 Seed I. GERM SIZE: 2 (1 =Small, 2 = Midsize, 3 = Large) 14. DISEASE: (0 = Not tested, 1 =Susceptible, 2 = Resistant, 3 = Intermediate, 4 = Tolerant) SPECIFICRACE OR STRAIN TESTED Stem Rust (Puccinia graminis f. sp.tritici) 0 LeafRust (Puccinia recondita f. sp. tritici) 2 Stripe Rust (Pucciniastriiformis) 2 Loose Smut (Ustilago tritici) 0 Powdery Mildew (Erysiphegraminis f. sp. tritici) 2 Common Bunt (Tilletia tritici or T. laevis) 0Dwarf Bunt (Tilletia controversa) 0 Karnal Bunt (Tilletia indica) 0 FlagSmut (Urocystis agropyri) 0 Tan Spot (Pyrenophora tritici-repentis) 0Halo Spot (Selenophoma donacis) 0 Septoria spp. 0 Septoria nodorum(Glume Blotch) 0 Septoria avenae (Speckled Leaf Disease) 0 Septoriatritici (Speckled Leaf Blotch) 0 Scab (Fusarium spp.) 3 “Snow Molds” 0Kernel Smudge (“Black Point”) 0 Common Root Rot (Fusarium, Cochliobolusand Bipolaris spp.) 0 Barley Yellow Dwarf Virus (BYDV) 0 RhizoctoniaRoot Rot (Rhizoctonia solani) 0 Soilborne Mosaic Virus (SBMV) 2 BlackChaff (Xanthomonas campestris pv. translucens). 0 Wheat Yellow (SpindleStreak) Mosaic Virus 0 Bacterial Leaf Blight (Pseudomonas syringae pv.syringae) 0 Wheat Streak Mosaic Virus (WSMV) 0 15. INSECT: (0 = Nottested, 1 = Susceptible, 2 = Resistant, 3 = Intermediate, 4 = Tolerant)Stem Sawfly (Cephus spp.) 0 Cereal Leaf Beetle (Oulema melanopa) 0Russian Aphid (Diuraphis noxia) 0 Greenbug (Schizaphis graminum) 0Aphids 0 Hessian Fly (Mayetiola destructor) Biotype L 2

In one aspect, wheat plants, plant parts and seeds are provided whichhave all or essentially all of the characteristics set forth in Table1B. In one aspect wheat plants, plant parts and seeds are provided whichhave all or essentially all of the physiological and morphologicalcharacteristics of wheat variety W050216N2, representative seed havingbeen deposited with the ATCC as disclosed herein.

Wheat variety W050216N2 can be further reproduced by tissue culture andregeneration. Tissue culture of various tissues of wheat andregeneration of plants therefrom is well known and widely published.Thus, in another aspect provided are cells which upon growth anddifferentiation produce wheat plants capable of having the physiologicaland morphological characteristics of wheat variety W050216N2.

As used herein, the term “plant parts” includes, without limitation,plant protoplasts, plant cell tissue cultures from which wheat plantscan be regenerated, plant calli, plant clumps, plant cells, embryos,pollen, ovules, pericarp, seed, flowers, florets, heads, spikes, leaves,roots, root tips, anthers, and the like. When indicating that a plant iscrossed or selfed this indicates that any plant part of the plant can beused. For instance the plant part does not need to be attached to theplant during the crossing or selfing, only the pollen might be used.

In one aspect, a wheat plant containing a locus conversion or anessentially derived variety of W050216N2 is provided. As determined bythe UPOV Convention, essentially derived varieties may be obtained forexample by the selection of a natural or induced mutant, or of asomaclonal variant, the selection of a variant individual from plants ofthe initial variety, backcrossing, or transformation by geneticengineering. An essentially derived variety of W050216N2 is furtherdefined as one whose production requires the repeated use of varietyW050216N2 or is predominately derived from variety W050216N2.International Convention for the Protection of New Varieties of Plants,as amended on Mar. 19, 1991, Chapter V, Article 14, Section 5(c).

A locus conversion refers to plants within a variety that have beenmodified in a manner that retains the overall genetics of the varietyand further comprises one or more loci with a specific desired trait,such as male sterility, insect, disease or herbicide resistance.Examples of single locus conversions include mutant genes, transgenesand native traits finely mapped to a single locus. One or more locusconversion traits may be introduced into a single wheat variety. As usedherein, the phrase ‘comprising a’ transgene, transgenic event or locusconversion means one or more transgenes, transgenic events or locusconversions.

Any DNA sequences, whether from a different species or from the samespecies that are inserted into the genome using transformation arereferred to herein collectively as “transgenes”. Methods for producingtransgenic plants and in particular embodiments, transformed versions ofthe wheat variety W050216N2 and methods for producing such plants areprovided.

Numerous methods for plant transformation have been developed, includingbiological protocols, such as using Agrobacterium, and physicaltechniques, such as biolistics and direct gene transfer. In addition,expression vectors and in vitro culture methods for plant cell or tissuetransformation and regeneration of plants are available. The mostprevalent types of plant transformation involve the construction of anexpression vector. Such a vector comprises a DNA sequence that containsa gene or coding sequence under the control of or operatively linked toa regulatory element, such as a promoter. The vector may contain one ormore genes or coding sequences and one or more regulatory elements. Theregulatory element or promoter can be heterologous to the gene or codingsequence.

Various elements can be introduced into the plant genome, including butare not limited to genes; coding sequences; inducible, constitutive, andtissue specific promoters; enhancing sequences; and signal and targetingsequences. As used herein, when used in transformation, a gene generallyreferences the coding sequence or cDNA which does not include intronsequences not encoding a polypeptide.

A genetic trait, engineered into a particular wheat plant usingtransformation techniques, can be moved into another line usingtraditional breeding techniques that are well known in the plantbreeding arts. For example, a backcrossing approach can be used to movea transgene from a transformed wheat plant to an elite wheat variety toprovide resulting progeny comprising a transgene. As used herein,“crossing” can refer to a simple X by Y cross, or the process ofbackcrossing, depending on the context. The term “breeding cross”excludes the processes of selfing or sibbing.

Transgenic wheat plants according to the present invention can beharvested to produce a foreign protein in commercial quantities. Theforeign protein can be extracted from a tissue of interest, such as aseed, or from total biomass by known methods. The approximatechromosomal location of the integrated DNA molecule can be determinedfrom a genetic map generated, for example, via conventional RFLP, PCR,and SSR analysis. Map information concerning chromosomal location isuseful for proprietary protection of a subject transgenic plant. Ifunauthorized propagation is undertaken and crosses made with othergermplasm, the map of the integration region can be compared to similarmaps for suspect plants, to determine if the latter have a commonparentage with the subject plant. Map comparisons would involvehybridizations, RFLP, PCR, SSR, SNPS and sequencing, all of which areconventional techniques.

As described herein, genes or coding sequences can be expressed intransformed plants. More particularly, plants can be geneticallyengineered to express various phenotypes of agronomic interest. A singlegene or locus conversion or at least about 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35 or 40 or moregenes or locus conversions and less than about 100, 90, 80, 70, 60, 50,40, 30, 20, 15, or 10 genes or locus conversions may be introduced intoa plant or comprised in the genome of the wheat plant. Combinations orstacks of two or more genes or coding sequences described herein can beused. Through the transformation of wheat the expression of genes can bemodulated to enhance disease resistance, insect resistance, herbicideresistance, water stress tolerance and agronomic traits as well as grainquality traits. These traits and the genes and organisms which may betargets are described in U.S. Pat. No. 8,809,554, which is incorporatedherein by reference in its entirety for this purpose. Transformation canalso be used to insert DNA sequences which control or help controlmale-sterility. DNA sequences native to wheat as well as non-native DNAsequences can be transformed into wheat and used to modulate levels ofnative or non-native proteins. The sequences can be heterologouscomprising a coding sequence operably linked to a heterologousregulatory element, such as a promoter. Anti-sense technology, variouspromoters, targeting sequences, enhancing sequences, and other DNAsequences can be inserted into the wheat genome for the purpose ofmodulating the expression of proteins.

Exemplary genes which can be used include, but are not limited to, genesthat confer resistance to pests such as Hessian fly, wheat stem sawfly,cereal leaf beetle, and/or green bug or disease, to pathogensCladosporium fulvum Pseudomonas syringae Fusarium graminearum Schwabe,wheat rusts, Septoria tritici, Septoria nodorum, powdery mildew,Helminthosporium diseases, smuts, bunts, Fusarium diseases, bacterialdiseases, and viral diseases.

Other genes, coding sequences or targets which can be used include thoseencoding Bacillus thuringiensis protein, a derivative thereof or asynthetic polypeptide modeled thereon. Examples of Bacillusthuringiensis transgenes encoding a endotoxin and being geneticallyengineered are given in the following patents and patent applicationsand hereby are incorporated by reference for this purpose: U.S. Pat.Nos. 5,188,960; 5,689,052; 5,880,275; 8,809,654; WO 91/14778; WO99/31248; WO 01/12731; WO 99/24581; WO 97/40162 and U.S. applicationSer. Nos. 10/032,717; 10/414,637; and Ser. No. 10/606,320.

Other genes, coding sequences or targets which can be used include thoseencoding an insect-specific hormone or pheromone such as an ecdysteroidand juvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof, an insect diuretic hormone receptor, suchas an allostatin (see also U.S. Pat. No. 5,266,317 incorporated hereinby reference for this purpose), an enzyme responsible for a hyperaccumulation of a monterpene, a sesquiterpene, a steroid, hydroxamicacid, a phenylpropanoid derivative or another non-protein molecule withinsecticidal activity, an enzyme involved in the modification, includingthe post-translational modification, of a biologically active molecule,for example, a glycolytic enzyme, a proteolytic enzyme, a lipolyticenzyme, a nuclease, a cyclase, a transaminase, an esterase, a hydrolase,a phosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic; a molecule thatstimulates signal transduction, for example mung bean calmodulin cDNAclones and maize calmodulin cDNA clones; a hydrophobic peptide (see U.S.Pat. No. 5,580,852 and U.S. Pat. No. 5,607,914 incorporated herein byreference for this purpose); a membrane permease, a channel former or achannel blocker, for example, cropin-beta lytic peptide analogconferring Pseudomonas solanacearum; an insect-specific antibody or animmunotoxin derived therefrom, or a virus-specific antibody; adevelopmental-arrestive protein such as aendopolygalacturonase-inhibiting protein or a ribosome-inactivatinggene; genes involved in the Systemic Acquired Resistance (SAR) Responseand/or the pathogenesis related genes,

In some embodiments, coat protein-mediated resistance can be conferredin plants against one or more of alfalfa mosaic virus, cucumber mosaicvirus, tobacco streak virus, potato virus X, potato virus Y, tobaccoetch virus, tobacco rattle virus and tobacco mosaic virus. Suchresistance may be conferred using, for example, a viral-invasive proteinor a complex toxin derived therefrom.

In some embodiments, genes, coding sequences or targets which can beused include, without limitation, antifungal genes (see, for example, USPublication No: 20020166141 incorporated herein by reference for thispurpose); detoxification genes, such as for fumonisin, beauvericin,moniliformin and zearalenone and their structurally related derivatives(see, for example, U.S. Pat. No. 5,792,931 incorporated herein byreference for this purpose); cystatin and cysteine proteinase inhibitors(see for example, US Patent Publication Serial No: 20050102717incorporated herein by reference for this purpose), defensin genes (seefor example, PCT Public WO03000863 and US Patent Publication Serial No:20030041348); and genes conferring resistance to nematodes, see forexample, WO 03/033651.

Genes, coding sequences, or targets that confer resistance to aherbicide are described, for example, in U.S. Pat. No. 8,809,654, whichis incorporated by reference herein for this purpose. Examples includegenes or coding sequences encoding acetohydroxy acid synthase, achimeric protein of rat cytochrome P4507A1, yeast NADPH-cytochrome P450oxidoreductase, glutathione reductase, superoxide dismutase,phosphotransferases, ALS and AHAS enzymes and other genes or codingsequences which confer resistance to a herbicide such as animidazalinone or a sulfonylurea (see also, U.S. Pat. Nos. 5,605,011;5,013,659; 5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373;5,331,107; 5,928,937; and 5,378,824; and international publication WO96/33270, each of which are incorporated herein by reference for thispurpose); Glyphosate or glufosinate resistance can also be conferredusing, for example, sequences encoding mutant5-enolpyruvl-3-phosphikimate synthase (EPSP), aroA genes,phosphinothricin acetyl transferase (PAT), glyphosate oxido-reductaseenzyme, glyphosate N-acetyltransferase, glutamine synthetase,Streptomyces hygroscopicus phosphinothricin acetyl transferase (bar)genes), and pyridinoxy or phenoxy proprionic acids and cycloshexones(ACCase inhibitor-encoding genes). See, for example, U.S. Pat. Nos.4,769,061, 4,975,374, 4,940,835, 5,776,760, 5,463,175, 5,627,061,6,566,587; 6,338,961; 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435;5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775;6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448;5,510,471; Re. 36,449; RE 37,287 E; and 5,491,288; US Patent PublicationNo. 20040082770 and international publications EP1173580; WO 01/66704;EP1173581 and EP1173582, EP 0 242 246 and EP 0 242 236, each of whichare incorporated herein by reference for this purpose. See also, U.S.Pat. Nos. 5,969,213; 5,489,520; 5,550,318; 5,874,265; 5,919,675;5,561,236; 5,648,477; 5,646,024; 6,177,616; and 5,879,903, each of whichare incorporated herein by reference for this purpose.

Triazine resistance can be conferred using, for example, psbA and gs+genes, sequences encoding a benzonitrile (nitrilase gene) such asdisclosed in U.S. Pat. No. 4,810,648 incorporated herein by referencefor this purpose.

Resistance to herbicides which target Protoporphyrinogen oxidase(protox) can also be conferred such resistance being described in U.S.Pat. Nos. 6,288,306, 6,282,837, 5,767,373 and international publicationWO 01/12825 the disclosures of each of which are herein incorporated byreference for this purpose.

Genes, coding sequences, or targets that confer or improve grain qualityinclude, without limitation, altered fatty acids (for example, oleic,linoleic, linolenic), altered phosphorus content (for example, usingphytase), altered carbohydrates such as modulating the branching patternof starch or altering thioredoxin, Bacillus subtilis levansucrase gene,Bacillus licheniformis alpha-amylase, tomato invertase, alpha-amylasegene, starch branching enzyme II, UDP-D-xylose 4-epimerase, Fragile 1and 2, Ref1, HCHL, C4H, high oil seed such as by modification of starchlevels (AGP). Fatty acid modification genes mentioned above may also beused to affect starch content and/or composition through theinterrelationship of the starch and oil pathways, altered content orcomposition of antioxidants such as tocopherol or tocotrienols, such asusing a phytl prenyl transferase (ppt), or through alteration of ahomogentisate geranyl geranyl transferase (hggt). Genes, codingsequences, or targets that can be targets to confer or improve grainquality are disclosed in, for example, see U.S. Pat. Nos. 8,809,654,6,787,683, 6,531,648, 6,423,886, 6,232,529, 6,197,561, 6,825,397, USPatent Publication Nos. 2003/0079247, US2003/0204870, US2004/0034886international PCT publications WO 02/42424, WO 98/22604, WO 03/011015,WO02/057439, WO03/011015, WO 99/10498, WO 00/68393, and WO 03/082899.

Genes, coding sequences or targets for altered essential seed aminoacids, such as one or more of lysine, methionine, threonine, tryptophanor altered sulfur amino acid content are also provided, can be used inthe methods and plants described herein and are described in, forexample, U.S. Pat. Nos. 8,809,654, 6,803,498, 6,127,600, 6,194,638,6,346,403, 6,080,913, 5,990,389, 5,939,599, 5,912,414, 5,850,016,5,885,802, 5,885,801, 5,633,436, 5,559,223, 6,664,445, 6,459,019,6,194,638, 6,399,859, 6,441,274, international PCT applicationsWO99/40209, WO99/29882, WO98/20133, WO96/01905, WO98/56935, WO98/45458,WO98/42831, WO95/15392, WO01/79516, WO00/09706, and US Publication Nos.US2003/0150014, US2003/0163838, US2004/0068767, and US2004/0025203, thedisclosures of each of which are herein incorporated by reference in itsentirety for these purposes.

Genes, coding sequences or targets that control or alter male sterilityand methods for conferring male sterility and male sterile plants areprovided. There are several methods of conferring genetic male sterilityavailable, such as disclosed in U.S. Pat. Nos. 8,809,654, 4,654,465 and4,727,219, 3,861,709, 3,710,511, 5,432,068, the disclosures of each ofwhich are herein incorporated by reference for this purpose. Foradditional examples of nuclear male and female sterility systems andgenes, see also, U.S. Pat. No. 5,859,341; U.S. Pat. No. 6,297,426; U.S.Pat. No. 5,478,369; U.S. Pat. No. 5,824,524; U.S. Pat. No. 5,850,014;and U.S. Pat. No. 6,265,640; each of which are hereby incorporated byreference for this purpose.

Genes, coding sequences or targets that create a site for site specificDNA integration can also be used such as the introduction of FRT sitesthat may be used in the FLP/FRT system and/or Lox sites that may be usedin the Cre/Loxp system. Other systems that may be used include the Ginrecombinase of phage Mu, the Pin recombinase of E. coli, and the R/RSsystem of the pSR1 plasmid.

Genes that affect abiotic stress resistance (including but not limitedto flowering, ear and seed development, enhancement of nitrogenutilization efficiency, altered nitrogen responsiveness, droughtresistance or tolerance, cold resistance or tolerance, and saltresistance or tolerance) and increased yield under stress are provided.For example, see: U.S. Pat. Nos. 8,809,654, 5,892,009, 5,965,705,5,929,305, 5,891,859, 6,417,428, 6,664,446, 6,706,866, 6,717,034,6,801,104, 6,177,275, 6,107,547, 6,084,153, US Patent Publication Nos.2004/0148654, 2004/0237147, 2003/0166197, 2004/0128719, 2004/0098764,2004/0078852, international PCT application WO2000060089, WO2001026459,WO2001035725, WO 00/73475; WO2001034726, WO2001035727, WO2001036444,WO2001036597, WO2001036598, WO2002015675, WO2002017430, WO2002077185,WO2002079403, WO2003013227, WO2003013228, WO2003014327, WO2004031349,WO2004076638, WO9809521, WO01/36596 and WO9938977, WO2000/006341,WO04/090143, WO0202776, WO2003052063, WO0164898, and WO200032761, thedisclosures of each of which are herein incorporated by reference in itsentirety for this purpose.

Other genes and transcription factors that affect plant growth andagronomic traits such as yield, flowering, plant growth and/or plantstructure, can be introduced or introgressed into plants, see e.g.WO97/49811 (LHY), WO98/56918 (ESD4), WO97/10339 and U.S. Pat. No.6,573,430 (TFL), U.S. Pat. No. 6,713,663 (FT), WO96/14414 (CON),WO96/38560, WO01/21822 (VRN1), WO00/44918 (VRN2), WO99/49064 (GI),WO00/46358 (FRI), WO97/29123, U.S. Pat. No. 6,794,560, U.S. Pat. No.6,307,126 (GAI), WO99/09174 (D8 and Rht), and WO2004076638 andWO2004031349 (transcription factors), the disclosures of each of whichare herein incorporated by reference.

Genes that confer agronomic enhancements, nutritional enhancements, orindustrial enhancements can also be used. Such genes are described forexample in U.S. Pat. No. 8,809,654, the disclosure of which is hereinincorporated by reference in for this purpose. Such enhancementsinclude, without limitation, improved tolerance to water stress fromdrought or high salt water condition. See e.g. U.S. Pat. Nos. 5,981,842,5,780,709, international patent applications WO 92/19731, WO 92/19731the disclosures of each of which is herein incorporated by reference forthis purpose.

In some embodiments, methods of treating W050216N2 with a mutagen andthe plant produced by mutagenesis of W050216N2 are provided. Backcrossconversions of wheat variety W050216N2 are also described. A backcrossconversion occurs when DNA sequences are introduced through traditional(non-transformation) breeding techniques, such as backcrossing. DNAsequences, whether naturally occurring or transgenes, may be introducedusing these traditional breeding techniques. Desired traits transferredthrough this process include, but are not limited to, nutritionalenhancements, industrial enhancements, disease resistance, insectresistance, herbicide resistance, agronomic enhancements, grain qualityenhancement, waxy starch, breeding enhancements, seed productionenhancements, and male sterility. Descriptions of some of thecytoplasmic male sterility genes, nuclear male sterility genes, chemicalhybridizing agents, male fertility restoration genes, and methods ofusing the aforementioned are discussed in “Hybrid Wheat by K. A. Lucken(pp. 444-452 In Wheat and Wheat Improvement, ed. Heyne, 1987). Examplesof genes for other traits which can be used with the methods, plants andplant parts described herein include: Leaf rust resistance genes (Lrseries such as Lr1, Lr10, Lr21, Lr22, Lr22a, Lr32, Lr37, Lr41, Lr42, andLr43), Fusarium head blight-resistance genes (QFhs.ndsu-3B andQFhs.ndsu-2A), Powdery Mildew resistance genes (Pm21), common buntresistance genes (Bt-10), and wheat streak mosaic virus resistance gene(Wsm1), Russian wheat aphid resistance genes (Dn series such as Dn1,Dn2, Dn4, Dn5), Black stem rust resistance genes (Sr38), Yellow rustresistance genes (Yr series such as Yr1, YrSD, Yrsu, Yr17, Yr15, YrH52),Aluminum tolerance genes (Alt(BH)), dwarf genes (Rht), vernalizationgenes (Vrn), Hessian fly resistance genes (H9, H10, H21, H29), graincolor genes (R/r), glyphosate resistance genes (EPSPS), glufosinategenes (bar, pat) and water stress tolerance genes (Hva1, mtlD). Thetrait of interest is transferred from the donor parent to the recurrentparent, in this case, the wheat plant disclosed herein. Single genetraits may result from either the transfer of a dominant allele or arecessive allele. Selection of progeny containing the trait of interestis done by direct selection for a trait associated with a dominantallele. Selection of progeny for a trait that is transferred via arecessive allele requires growing and selfing the first backcross todetermine which plants carry the recessive alleles. Recessive traits mayrequire additional progeny testing in successive backcross generationsto determine the presence of the gene of interest.

Methods of developing a backcross conversion W050216N2 wheat plant areprovided including the step of repeated backcrossing to wheat varietyW050216N2. The number of backcrosses made may be 2, 3, 4, 5, 6, 7, 8 orgreater, and fewer than 50, 40, 30, 25, 20, 15, 10, 9, or 8. Thespecific number of backcrosses used will depend upon the genetics of thedonor parent and whether molecular markers are utilized in thebackcrossing program. Provided are plants and plant populations that areproduced from backcrossing methods, transformation, locus conversion, orotherwise produced, and combinations thereof and that retain at least70%, 75%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8%,or 99.9% or 99.95%, 99.98%, 99.985%, 99.99% or 99.995% of the geneticprofile of wheat variety W050216N2. The percentage of the geneticsretained in the backcross conversion may be measured by either pedigreeanalysis or through the use of genetic techniques such as molecularmarkers or electrophoresis. Such methods and techniques are described inU.S. Pat. No. 8,809,654, the disclosure of which is herein incorporatedby reference for this purpose. The backcross conversion or locusconversion developed from this method may be similar to W050216N2 forthe results listed in Table 1B. Such similarity may be measured by aside by side phenotypic comparison, with differences and similaritiesdetermined at a 5% significance level, when appropriate in environmentalconditions that account for the trait being transferred. For example,herbicide should not be applied in the phenotypic comparison ofherbicide resistant backcross conversion of W050216N2 when compared backto W050216N2.

Described are methods for using wheat variety W050216N2 in plantbreeding and plants and plant populations produced by such methods. Forexample, wheat variety W050216N2 can be crossed with another variety ofwheat to form a first generation population of F1 plants. This firstgeneration population of F1 plants will comprise an essentially completeset of the alleles of wheat variety W050216N2. Also provided are methodsand plants which use transgenic or backcross conversions of wheatvariety W050216N2 to produce first generation F1 plants.

A method of developing a W050216N2-progeny wheat plant comprisingcrossing W050216N2 with a second wheat plant and performing a breedingmethod is also described. An exemplary method for producing a linederived from wheat variety W050216N2 is as follows. Wheat varietyW050216N2 is crossed with another variety of wheat, such as an elitevariety. The F1 seed derived from this cross is grown to form ahomogeneous population. The F1 seed contains one set of the alleles fromvariety W050216N2 and one set of the alleles from the other wheatvariety. The F1 genome is 50% variety W050216N2 and 50% of the otherelite variety. The F1 seed is grown and allowed to self, thereby formingF2 seed. On average the F2 seed would have derived 50% of its allelesfrom variety W050216N2 and 50% from the other wheat variety, but variousindividual plants from the population can have a much greater percentageof their alleles derived from W050216N2. The F2 seed is grown andselection of plants made based on visual observation and/or measurementof traits. The W050216N2-derived progeny that exhibit one or more of thedesired W050216N2-derived traits are selected and each plant isharvested separately. This F3 seed from each plant is grown inindividual rows and allowed to self. Then selected rows or plants fromthe rows are harvested and threshed individually. The selections basedon visual observation and/or measurements for desirable traits of theplants, such as one or more of the desirable W050216N2-derived traitsare made. The process of growing and selection is repeated any number oftimes until a homozygous W050216N2-derived wheat plant is obtained. Thehomozygous W050216N2-derived wheat plant contains desirable traitsderived from wheat variety W050216N2, some of which may not have beenexpressed by the other original wheat variety to which wheat varietyW050216N2 was crossed and some of which may have been expressed by bothwheat varieties but now would be at a level equal to or greater than thelevel expressed in wheat variety W050216N2. The homozygousW050216N2-derived wheat plants have, on average, 50% of their genesderived from wheat variety W050216N2, but various individual plants fromthe population would have a much greater percentage of their allelesderived from W050216N2. The breeding process, of crossing, selfing, andselection may be repeated to produce another population ofW050216N2-derived wheat plants with, on average, 25% of their genesderived from wheat variety W050216N2, and with various individual plantsfrom the population having a much greater percentage of their allelesderived from W050216N2. Homozygous W050216N2-derived wheat plants thathave received W050216N2-derived traits are also provided.

In some instances, selection may or may not occur at every selfinggeneration, selection may occur before or after the actualself-pollination process occurs, or individual selections may be made byharvesting individual spikes, plants, rows or plots at any point duringthe breeding process described herein. In addition, double haploidbreeding methods may be used at any step in the process. In one aspect,the population of plants produced at each and any generation of selfing,each such population consisting of plants containing approximately 50%of its genes from wheat variety W050216N2, 25% of its genes from wheatvariety W050216N2 in the second cycle of crossing, selfing, andselection, 12.5% of its genes from wheat variety W050216N2 in the thirdcycle of crossing, selfing, and selection, and so on.

Also disclosed are methods of obtaining a homozygous W050216N2-derivedwheat plant by crossing wheat variety W050216N2 with another variety ofwheat and applying double haploid methods to the F1 seed or F1 plant orto any generation of W050216N2-derived wheat obtained by the selfing ofthis cross.

Still further, methods for producing W050216N2-derived wheat plants areprovided by crossing wheat variety W050216N2 with a wheat plant andgrowing the progeny seed, and repeating the crossing or selfing alongwith the growing steps with the W050216N2-derived wheat plant from 1 to2 times, 1 to 3 times, 1 to 4 times, or 1 to 5 times. Thus, any and allmethods using wheat variety W050216N2 in breeding, including selfing,pedigree breeding, backcrossing, hybrid production and crosses topopulations are provided. Unique starch profiles, molecular markerprofiles and/or breeding records can be used to identify the progenylines or populations derived from these breeding methods.

Also disclosed are methods of harvesting the grain of variety wheatvariety W050216N2 and using the grain as seed for planting. Embodimentsinclude cleaning the seed, treating the seed, and/or conditioning theseed. Cleaning the seed includes removing foreign debris such as weedseed and removing chaff, plant matter, from the seed. Conditioning theseed can include controlling the temperature and rate of dry down andstoring seed in a controlled temperature environment. Seed treatment isthe application of a composition to the seed such as a coating orpowder. Seed material can be treated, typically surface treated, with acomposition comprising combinations of chemical or biologicalherbicides, herbicide safeners, pesticides, insecticides, fungicides,nutrients, germination inhibitors, germination promoters, cytokinins,nutrients, plant growth regulators, antimicrobials, and activators,bactericides, nematicides, avicides, or molluscicides. These compoundsare typically formulated together with further carriers, surfactants orapplication-promoting adjuvants customarily employed in the art offormulation. The coatings may be applied by impregnating propagationmaterial with a liquid formulation or by coating with a combined wet ordry formulation. Examples of the various types of compounds that may beused as seed treatments are provided in The Pesticide Manual: A WorldCompendium, C.D.S. Tomlin Ed., published by the British Crop ProductionCouncil. Some specific seed treatments that may be used on crop seedinclude, but are not limited to, abscisic acid, acibenzolar-S-methyl,avermectin, amitrol, azaconazole, azospirillum, azoxystrobin, bacillus,Bacillus subtilis, Bacillus simplex, Bacillus firmus, Bacillusamyloliquefaciens, Pasteuria genus (e.g. P. nishizawae), bradyrhizobium,captan, carboxin, chitosan, clothianidin, copper, cyazypyr,difenoconazole, etidiazole, fipronil, fludioxonil, fluquinconazole,flurazole, fluxofenim, GB126, Harpin protein, imazalil, imidacloprid,ipconazole, isofavenoids, lipo-chitooligosaccharide, mancozeb,manganese, maneb, mefenoxam, metalaxyl, metconazole, PCNB, penflufen,penicillium, penthiopyrad, permethrine, picoxystrobin, prothioconazole,pyraclostrobin, rynaxypyr, S-metolachlor, saponin, sedaxane, TCMTB,tebuconazole, thiabendaxole, thiamethoxam, thiocarb, thiram,tolclofos-methyl, triadimenol, trichoderma, trifloxystrobin,triticonazole and/or zinc.

It is routine practice to test seed varieties and seeds with specificgenetic resistance traits to determine which seed treatment options andapplication rates will complement such varieties and genetic resistancetraits in order to enhance yield. For example, a variety with good yieldpotential but loose smut susceptibility will benefit from the use of aseed treatment that provides protection against loose smut. Likewise, avariety encompassing a genetic resistance trait conferring insectresistance will benefit from the second mode of action conferred by theseed treatment. Further, the good root establishment and early emergencethat results from the proper use of a seed treatment will result in moreefficient nitrogen use, a better ability to withstand drought and anoverall increase in yield potential of a variety or varieties containinga certain trait when combined with a seed treatment.

Methods for analyzing polynucleotides from plants, plant parts or seedsdescribed herein may include contacting a polynucleotide from the plant,plant part or seed, such as from wheat variety W050216N2 with amolecular marker or with modified nucleotides that facilitate sequencingof the polynucleotide. The polynucleotide may be isolated, separated orotherwise obtained from the plant, plant part or seed. Modifiednucleotides such as dNTPs may be incorporated with the polynucleotidesalong with appropriate primers in a reaction mixture that facilitatessequencing. Sequencing can be done using any method known in the art.

It will be apparent to those of skill in the art that variations may beapplied to the compositions and methods described herein and in thesteps or in the sequence of steps of the method described herein withoutdeparting from the concept, spirit and scope of the invention. Morespecifically, it will be apparent that certain changes and modificationssuch as single gene conversions, including for example, modificationsand mutations, somoclonal variants, variant individuals selected fromlarge populations of the plants of the instant inbred and the like maybe practiced. All such similar substitutes and modifications apparent tothose skilled in the art are deemed to be within the spirit, scope andconcept of the invention.

It is to be understood that the invention is not limited in itsapplication to the details of construction and the arrangement ofcomponents set forth in the following description. Also, it is to beunderstood that the phraseology and terminology used herein is for thepurpose of description and should not be regarded as limiting. The useof “including,” “comprising,” or “having” and variations thereof hereinis meant to encompass the items listed thereafter and equivalentsthereof as well as additional items.

It also is understood that any numerical range recited herein includesall values from the lower value to the upper value. For example, if aconcentration range is stated as 1% to 50%, it is intended that valuessuch as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expresslyenumerated in this specification. These are only examples of what isspecifically intended, and all possible combinations of numerical valuesbetween and including the lowest value and the highest value enumeratedare to be considered to be expressly stated in this application.

As used in this specification and the appended claims, the singularforms “a,” “an,” and “the” include plural referents unless the contentclearly dictates otherwise. It should also be noted that the term “or”is generally employed in its sense including “and/or” unless the contentclearly dictates otherwise. All publications, patents and patentapplications are herein expressly incorporated by reference in theirentirety for all purposes to the same extent as if each individualpublication or patent application was specifically and individuallyincorporated by reference. In case of conflict between the presentdisclosure and the incorporated patents, publications and references,the present disclosure should control.

EXAMPLES Example 1 Performance of W050216N2

In the examples that follow, the traits and characteristics of wheatvariety W050216N2 are given in paired comparisons with another varietyduring the same growing conditions and same year. The data collected oneach wheat variety is presented for a number of characteristics andtraits (Table 2, Table 3, and Table 4).

The results in Table 2 compare variety W050216N2 to varieties 25R47,25R32, and 25R62 for various agronomic traits. Data in Table 2 wascollected at locations in Illinois, Indiana, Michigan, Missouri, Ohio,and Ontario, Canada. The results in Table 3 compare variety W050216N2 tovarieties 25R47, 25R32, and 25R62 for various disease resistance traits.Data in Table 3 was collected at locations in Illinois, Indiana,Michigan, Missouri, Ohio, and Ontario, Canada. The results in Table 4show values for the grain quality of variety W050216N2 and comparisonvarieties 25R47, 25R32, and 25R62. Quality data were collected from2012-2013 at the USDA ARS Soft Wheat Quality Lab in Wooster, Ohio.

TABLE 2 Agronomic trait paired comparisons of W050216N2 during theperiod 2011-2013. Heading Grain Test Date Winter Plant Straw YieldWeight After hardiness Height Lodging Variety bu/ac lb/bu Jan 1 1-9@ cm1-9@ 2011-2013 W050216N2 95.3 58.4 126.0 4.0 94.0 5.0 25R47 97.7 56.9126.0 7.0 86.4 7.0 Locations 28 28 4 2 5 3 Reps. 50 50 8 4 9 5 Prob.0.1544 0.0000 0.4444 0.2716 0.0032 0.3356 2011-2013 W050216N2 95.3 58.4126.0 4.0 94.0 5.0 25R32 93.9 59.2 126.0 5.0 88.9 5.0 Locations 28 28 42 5 3 Reps. 50 50 8 4 9 5 Prob. 0.4425 0.0026 0.8614 0.2048 0.03990.8399 2013 W050216N2 97.8 57.5 137.0 4.0 91.4 5.0 25R62 102.7 55.0135.0 6.0 83.8 4.0 Locations 16 16 2 2 3 2 Reps. 32 32 4 4 6 4 Prob.0.0499 0.0000 0.2048 0.2578 0.0091 0.2048 @Scale of 1-9 where 9 =excellent or resistant, 1 = poor or susceptible.Data in above table collected at locations in Illinois, Indiana,Michigan, Missouri, Ohio, and Ontario, Canada.

TABLE 3 Disease trait paired comparisons of W050216N2 during the period2011-2013. Leaf Stripe Powdery Scab Rust Rust Mildew SBMV Variety 1-9@1-9@ 1-9@ 1-9@ 1-9@ 2011-2013 W050216N2 7.0 8.0 8.0 9.0 8.0 25R47 4.04.0 8.0 4.0 6.0 Locations 5 5 5 1 1 Reps. 9 8 9 2 2 Prob. 0.0047 0.00360.0890 2011-2013 W050216N2 7.0 8.0 8.0 9.0 8.0 25R32 7.0 3.0 8.0 8.0 8.0Locations 5 5 5 1 1 Reps. 9 8 9 2 2 Prob. 0.6213 0.0008 0.1778 2013W050216N2 7.0 8.0 9.0 9.0 8.0 25R62 6.0 3.0 9.0 7.0 8.0 Locations 3 3 11 1 Reps. 6 5 2 2 2 Prob. 0.1181 1.0000 @Scale of 1-9 where 9 =excellent or resistant, 1 = poor or susceptible. SBMV= Soil-borne MosaicVirus.Data in above table collected at locations in Illinois, Indiana,Michigan, Missouri, Ohio, and Ontario, Canada.

TABLE 4 Average Soft wheat quality data, 2012-2013. Break Lactic FlourYield Flour Yield Flour Protein Sucrose SRC Acid SRC Variety % % % % %2012-2013 W050216N2 69.3 37.5 7.6 90.5 79.9 25R47 70.5 42.7 6.5 84.982.4 Years 2 2 2 2 2 Reps. 2 2 2 2 2 Prob. 0.5827 0.3950 0.1644 0.35970.7225 2013 W050216N2 67.1 32.1 7.6 97.6 87.1 25R32 67.4 24.3 8.2 93.8103.1 Years 1 1 1 1 1 Reps. 1 1 1 1 1 Prob. 2013 W050216N2 67.1 32.1 7.697.6 87.1 25R62 68.8 36.5 6.5 91.8 81.6 Years 1 1 1 1 1 Reps. 1 1 1 1 1Prob. Lactic Acid SRC = Lactic Acid Solvent Retention Capacity SucroseSRC = Sucrose solution Retention Capacity Quality data collected at theUSDA-ARS Soft Wheat Quality Lab in Wooster, OHLactic Acid SRC=Lactic Acid Solvent Retention CapacitySucrose SRC=Sucrose solution Retention CapacityQuality data collected at the USDA-ARS Soft Wheat Quality Lab inWooster, Ohio

Examples 2-13 Assays Performed to Develop W050216N2

The following examples provide descriptions of several assays that canbe used to characterize and/or select a wheat variety during one or morestages of variety development. Many other methods and assays areavailable and can be substituted for, or used in combination with, oneor more of the examples provided herein. Tables 1, 2, 3 and 4 providefurther information on wheat variety W050216N2, which results may beproduced from at least one or more assays or methods described in thefollowing examples.

Example 2 Stripe Rust Screening

Stripe rust is a fungal leaf disease that is most common in themid-southern United States in the early spring. Significant levels ofthe disease can be found in some seasons anywhere in North America. Theinfection often mostly occurs on the flag leaf but it may attack theentire plant, including the head. Natural infection of plants in thefield may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Some majorgenes for resistance may be detected using controlled seedling screeningexperiments inoculated with specific races of the pathogen. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 3 Leaf Rust Screening

Leaf rust is a fungal leaf disease that is most common in the southernUnited States in the spring and early summer. Significant levels of thedisease can be found in most seasons anywhere in North America. Theinfection is most damaging when it occurs on the flag leaf but it mayattack the entire plant, including the head. Natural infection of plantsin the field may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Some majorgenes for resistance may be detected using controlled seedling screeningexperiments inoculated with specific races of the pathogen. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 4 Leaf Blight Screening

Fungal leaf blights, including Tan spot, Septoria tritici blotch, andStagnospora nodorum blotch, are common in much of the North Americanwheat growing regions. The infection is most damaging when it occurs onthe flag leaf but it may attack the entire plant, including the head.Natural infection of plants in the field may be rated visually using a1-9 scale, where 1 indicates complete susceptibility and 9 indicatescomplete resistance.

Example 5 Scab Screening

Fusarium head blight or scab is a fungal disease that is common in muchof the North American wheat growing regions. Infection occurs duringflowering and is most severe when conditions are wet, warm and remainhumid. The disease infects flowers on the spike and will spread toadjacent flowers, often infecting most of the developing kernels on thespike. Natural infection of plants in the field may be rated visuallyusing a 1-9 scale, where 1 indicates complete susceptibility and 9indicates complete resistance. Infection may be induced in controlledscreening experiments where spikes are inoculated with specific sporeconcentrations of the fungus by spraying the spikes at flowering orinjecting the inoculum directly into a flower on each spike. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 6 Powdery Mildew Screening

Powdery mildew is a fungal leaf disease that is most common in thesouthern United States in the spring and early summer. Significantlevels of the disease can be found in many seasons anywhere in NorthAmerica. The infection is most damaging when it occurs on the flag leafbut it may attack the entire plant, including the head. Naturalinfection of plants in the field may be rated visually using a 1-9scale, where 1 indicates complete susceptibility and 9 indicatescomplete resistance. Some major genes for resistance may be detectedusing controlled seedling screening experiments inoculated with specificraces of the pathogen. There are also molecular markers for QTL linkedto some specific resistance genes.

Example 7 Soilborne Mosaic Virus Screening

Soilborne mosaic virus is transmitted by the vector, Polymyxa graminis,which tends to be most common in low-lying, wet soils; particularlythose frequently grown to wheat. Symptoms appear in the spring as lightgreen to yellow mottling along with stunting and resetting plant growthin the most susceptible varieties. Natural infection of plants in thefield may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Higherlevels of natural infection can be induced for screening by plantingwheat annually in the same field to increase the vector level.

Example 8 Wheat Yellow (Spindle Streak) Mosaic Virus Screening

Wheat yellow virus is transmitted by the vector, Polymyxa graminis, andis most common during cool weather conditions in the spring. Symptomsappear as light green to yellow streaks and dashes parallel to the leafveins. Symptoms often fade prior to heading as weather conditions becomewarmer. Natural infection of plants in the field may be rated visuallyusing a 1-9 scale, where 1 indicates complete susceptibility and 9indicates complete resistance.

Example 9 Flour Yield Screening

The potential average flour yield of wheat can be determined on samplesof grain that has been cleaned to standard and tempered to uniformmoisture, using a test mill such as the Allis-Chalmers or Brabendermill. Samples are milled to established parameters, the flour siftedinto fractions, which are then weighed to calculate flour yield as apercentage of grain weight.

Flour yield “as is” is calculated as the bran weight (over 40 weight)subtracted from the grain weight, divided by grain weight and times 100to equal “as is” flour yield. Flour yield is calculated to a 15% grainmoisture basis as follows: flour moisture is regressed to predict thegrain moisture of the wheat when it went into the Quad Mill using theformulaInitial grain moisture=1.3429×(flour moisture)−4.The flour yields are corrected back to 15% grain moisture afterestimating the initial grain moisture using the formulaFlour Yield_((15%))=Flour Yield_((as is))−1.61%×(15%−Actual flourmoisture)

Example 10 Flour Protein Screening

The protein content as a percentage of total flour may be estimated bythe Kjeldahl method or properly calibrated near-infrared reflectanceinstruments to determine the total nitrogen content of the flour.

Flour protein differences among cultivars can be a reliable indicator ofgenetic variation provided the varieties are grown together, but canvary from year to year at any given location. Flour protein from asingle, non-composite sample may not be representative. Based on theSoft Wheat Quality Laboratory grow-outs, protein can vary as much 1.5%for a cultivar grown at various locations in the same ½ acre field.

Example 11 Sucrose Solvent Retention Capacity (SRC)

The solvent retention capacity (SRC) of wheat flour measures the abilityof the flour to retain various solvents after centrifugation. SucroseSRC predicts the starch damage and pentosan components, and can becorrelated to sugar-snap cookie diameter quality metrics.

Sucrose SRC is a measure of arabinoxylans (also known as pentosans)content, which can strongly affect water absorption in baked products.Water soluble arabinoxylans are thought to be the fraction that mostgreatly increases sucrose SRC. Sucrose SRC probably is the bestpredictor of cookie quality, with sugar snap cookie diameters decreasingby 0.07 cm for each percentage point increase in sucrose SRC. Thenegative correlation between wire-cut cookie and sucrose SRC values isr=−0.66 (p<0.0001). Sucrose SRC typically increases in wheat sampleswith lower flour yield (r=−0.31) and lower softness equivalent(r=−0.23). The cross hydration of gliadins by sucrose also causessucrose SRC values to be correlated to flour protein (r=0.52) and lacticacid SRC (r=0.62). Soft wheat flours for cookies typically have a targetof 95% or less when used by the US baking industry for biscuits andcrackers. Sucrose SRC values increase by 1% for every 5% increase inlactic acid SRC. The 95% target value can be exceeded in flour sampleswhere a higher lactic acid SRC is required for product manufacture sincethe higher sucrose SRC is due to gluten hydration and not to swelling ofthe water soluble arabinoxylans.

Example 12 Lactic Acid SRC

Lactic Acid SRC=Lactic Acid Solvent Retention Capacity. Lactic acid SRCmeasures gluten strength. Typical values are below 85% for “weak” softvarieties and above 105% or 110% for “strong” gluten soft varieties. Seethe above discussion of protein quality in this section for additionaldetails of the lactic acid SRC. Lactic acid SRC results correlate to theSDS-sedimentation test. The lactic acid SRC is also correlated to flourprotein concentration, but the effect is dependent on genotypes andgrowing conditions. The SWQL typically reports a protein-correctedlactic acid SRC value to remove some of the inherent protein fluctuationnot due to cultivar genetics. Lactic acid is corrected to 9% proteinusing the assumption of a 7% increase in lactic acid SRC for every 1%increase in flour protein. On average across 2007 and 2008, the changein lactic acid SRC value was closer to 2% for every 1% protein.

Example 13 Molecular Screening

As shown in Table 1B, plants were analyzed at various times throughoutthe development of W050216N2 for specific alleles for scab resistance.As discussed above, and as is known to those skilled in the art, othertraits can also be screened by molecular analysis.

Deposit

Applicant has made a deposit of at least 2500 seeds of Wheat VarietyW050216N2 with the American Type Culture Collection (ATCC), 10801University Boulevard, Manassas, Va. 20110 USA, ATCC Deposit No.PTA-123675. The seeds deposited with the ATCC on Dec. 6, 2016 were takenfrom the seed stock maintained by Pioneer Hi-Bred International, Inc.,7250 NW 62^(nd) Avenue, Johnston, Iowa, 50131 since prior to the filingdate of this application. Access to this seed will be available duringthe pendency of the application to the Commissioner of Patents andTrademarks and persons determined by the Commissioner to be entitledthereto upon request. Upon allowance of any claims in the application,the Applicant will make the deposit available to the public pursuant to37 C.F.R. §1.808. This deposit of the Wheat Variety W050216N2 will bemaintained in the ATCC depository, which is a public depository, for aperiod of 30 years, or 5 years after the most recent request, or for theenforceable life of the patent, whichever is longer, and will bereplaced if it becomes nonviable during that period. Additionally,Applicant has or will satisfy all of the requirements of 37 C.F.R.§§1.801-1.809, including providing an indication of the viability of thesample upon deposit. Applicant has no authority to waive anyrestrictions imposed by law on the transfer of biological material orits transportation in commerce. Applicant does not waive anyinfringement of rights granted under this patent or under the PlantVariety Protection Act (7 USC 2321 et seq.). Unauthorized seedmultiplication is prohibited.

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding.However, it will be obvious that certain changes and modifications suchas single locus modifications and mutations, somaclonal variants,variant individuals selected from large populations of the plants of theinstant variety and the like may be practiced.

The scope of the claims should not be limited by the preferredembodiments set forth in the examples, but should be given the broadestinterpretation consistent with the description as a whole.

All publications, patents and patent applications mentioned in thespecification are indicative of the level of those skilled in the art towhich this invention pertains.

What is claimed is:
 1. A plant, plant part, seed, or plant cell of wheatvariety W050216N2, representative seed of said variety having beendeposited under ATCC accession number PTA-123675.
 2. A wheat seedproduced from the crossing of the plant or plant part of claim 1 with adifferent wheat plant or plant part.
 3. A wheat plant or plant partproduced by growing the wheat seed of claim
 2. 4. A method for producinga second wheat plant comprising applying plant breeding techniques tothe wheat plant or plant part of claim 3, wherein application of saidtechniques results in the production of a second wheat plant.
 5. Amethod for producing a progeny seed comprising crossing the wheat plantof claim 3, to a plant of wheat variety W050216N2, representative seedof said variety having been deposited under ATCC accession numberPTA-123675 and producing a progeny seed.
 6. The method of claim 5further comprising crossing a plant grown from the progeny seed of claim5 to a plant of wheat variety W050216N2 and producing a backcrossedseed.
 7. The backcrossed seed produced by claim
 6. 8. A method forproducing a double haploid wheat plant or plant part comprising a)crossing the wheat plant of claim 3, to another plant to form haploidcells; b) doubling the chromosomes of said haploid cells to form doublehaploid cells; and c) growing said double haploid cells into a doublehaploid wheat plant or plant part.
 9. A method comprising cleaning theseed of claim
 1. 10. A method comprising conditioning the seed ofclaim
 1. 11. A method comprising applying a seed treatment to the seedof claim
 1. 12. Flour produced by milling the seed of claim
 1. 13. Atissue culture of cells produced from the plant, plant part, seed, orplant cell of claim
 1. 14. A wheat plant regenerated from the tissueculture of claim
 13. 15. A wheat plant comprising a transgene whereinsaid wheat plant was produced by transforming the plant, plant part,seed, or cell of claim
 1. 16. A plant, plant part, seed, or plant cellof wheat variety W050216N2, representative seed of said variety havingbeen deposited under ATCC accession number PTA-123675, furthercomprising a locus conversion.
 17. The plant, plant part, seed, or plantcell of claim 16, wherein the locus conversion confers a trait selectedfrom the group consisting of male sterility, abiotic stress tolerance,altered phosphorus, altered antioxidants, altered fatty acids, alteredessential amino acids, altered carbohydrates, herbicide resistance,insect resistance and disease resistance.
 18. A wheat seed produced bycrossing the plant of claim 16 with a different wheat plant.
 19. A wheatplant produced by growing the wheat seed of claim
 18. 20. A method forproducing a second wheat plant comprising applying plant breedingtechniques to the wheat plant of claim 19, wherein application of saidtechniques results in the production of a second wheat plant.